This is not quite what comes to mind when one thinks of
archaeological discoveries. The
examination of ancient poop t is one way that DNA from thousands of years ago
can be recovered. The proper archaeological term for the study of poop is called
the study of paleofeces or non-hardened fossils. Paleofeces are studied to determine how the
people of the time ate, what their diet consisted of, their health, and even
more about the plant and animal life that existed around them. Yes, all this can be found in the DNA of
poop.
The process of recovering DNA from paleofeces involves a simple
five step procedure involving liquid nitrogen, a diabetic drug, a polymerase
chain reaction machine, and poop. The nitrogen freezes the poop so it can be
ground into a fine powder, the diabetic drug is used to break down the sugar compounds
in the surrounding DNA, and the chain reaction machine is used to make millions
of copies of the recovered DNA to be compared to other DNA fragments from different
sources found at the site.
The reason this is possible is because of the process known
as the Maillard reaction, named after Louis Camille Maillard . What happens is that as the feces dries
a hard crust forms on the outside . The sugars from the digested plant material
begin to react with surrounding amino acids forming larger sugar compounds. These basically encase the DNA preserving it
for future extraction.
It is pretty neat to think that so much information can be
found in paleofeces that is thousands of years old. It was Hendrik Poinar and Svante Paabo from
the Max Planck Institute in Munich who recovered the first successful DNA sample
from paleofeces in 1998. It was in 2008
that the first human DNA extraction was made by the Kristin Sobolik,
archeologist from the University of Maine Orono.
Just recently archaeologists have unearthed 700 year old barrels filled with poop in Denmark.
Unfortunately, the Maillard reaction had not occurred since the waste
was sealed in the barrels. There was no
sugar coating to keep the smell in. Yet
the discovery was remarkable and the waste found told a lot about the people
who lived in Demark 700 years ago. The
archaeologists just had to endure the smell.
I wonder if our society today, with all the waste treatment
plants, has inadvertently put an end to DNA sampling of our poop by future
scientists? Who knows, maybe each city
will create a time capsule with a little sample of poop to let future generations
know about how we existed.
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